Recombinant Rabbit anti-Human IgA Antibody

Catalog No. ABIN7477497

Product Details

Target: IgA

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Application: Immunohistochemistry (IHC)

Clone: MSVA-700R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong staining should be seen in a fraction of plasma cells.

Negative Control: Tonsil: Lymphocytes should not stain for IgA.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background: A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2,IgA antibody validated for immunohistochemistry on 76 different Normal Tissues