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Rabbit anti-Human IgA (Heavy Chain) Antibody (FITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG FITC
Catalog No. ABIN100725
$264.00
Plus shipping costs $45.00
1 mg
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  • Target
    IgA
    Binding Specificity
    Heavy Chain
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    FITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Human IgA alpha heavy chain

    Isotype
    IgG
    Labeling Ratio
    5.1
  • Application Notes

    Application Note: Anti-Human IgA Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgA
    Background

    Synonyms: rabbit anti-Human IgA (alpha chain) fluorescein conjugated Antibody, rabbit anti-Human IgA alpha Antibody FITC conjugation

    Background: Anti-Human IgA Fluorescein Antibody generated in rabbit detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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