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Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN100788
  • Target See all IgA, IgG, IgM products
    IgA, IgG, IgM
    Binding Specificity
    • 74
    • 58
    • 31
    • 16
    • 9
    Heavy & Light Chain
    Reactivity
    • 71
    • 47
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Human
    Host
    • 160
    • 55
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 218
    • 1
    Polyclonal
    Application
    • 116
    • 111
    • 76
    • 73
    • 68
    • 56
    • 26
    • 24
    • 23
    • 22
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG IgA IgM
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Human IgG, IgA and IgM whole molecules

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgG IgA IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:28,800 - 1:48,800

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgA, IgG, IgM
    Alternative Name
    IgG IgA IgM (IgA, IgG, IgM Products)
    Target Type
    Antibody
    Background

    Synonyms: Goat anti Human IgG IgA IgM Antibody, Goat Anti-Human IgG IgA IgM Antibody, Goat Anti-Human IgGAM

    Background: Anti-Human IgG IgA IgM (H&L) Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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