Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN100789

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG IgA IgM

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Human IgG, IgA and IgM whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Human IgG IgA IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:3,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG IgA IgM (IgA, IgG, IgM Products)

Target Type: Antibody

Background:

Synonyms: Goat Anti-Human IgG IgA IgM Antibody alkaline phosphatase Conjugated, Goat Anti-Human IgG IgA IgM Antibody alk phos Conjugated, Goat Anti-Human IgGAM Antibody ALP Conjugated

Background: Anti-Human IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.