Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Details for Product No. ABIN100790
Antigen
Epitope
Heavy & Light Chain
65
35
9
5
5
Reactivity
Human
60
33
20
15
11
9
4
4
3
3
3
2
2
1
1
Host
Goat
106
56
4
3
2
Clonality
Polyclonal
Conjugate
Biotin
28
27
17
14
9
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Human IgG, IgA and IgM whole molecules

Isotype IgG
Specificity IgG IgA IgM
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Antigen
Alternative Name IgG IgA IgM
Background

Synonyms: Goat anti Human IgG IgA IgM Biotin Conjugate Antibody, Goat Anti-Human IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Human IgGAM Biotin Conjugation

Background: Anti-Human IgG IgA IgM (H&L) Biotin Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

ELISA Dilution: 1:400,000

Western Blot Dilution: 1:5,000 - 1:20,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Aliquot to Avoid repeated freezing and thawing.
Storage RT,4 °C,-20 °C
Expiry Date 12 months