Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Details for Product No. ABIN100791
Antigen
Epitope
Heavy & Light Chain
65
35
9
5
5
Reactivity
Human
60
33
20
15
11
9
4
4
3
3
3
2
2
1
1
Host
Goat
106
56
4
3
2
Clonality
Polyclonal
Conjugate
FITC
28
26
18
14
9
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogen

Immunogen: Human IgG, IgA and IgM whole molecules

Isotype IgG
Specificity IgG IgA IgM
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using antigens coupled to agarose beads
Labeling Ratio 4.9
Antigen
Alternative Name IgG IgA IgM
Background

Synonyms: Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgGAM Antibody FITC Conjugated

Background: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) antibody (FITC) - Preadsorbed (ABIN100791) Dot Blot of Goat anti-Human IgG IgA IgM Antibody Fluorescein Conjugated. Antigen: Row...