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Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

FACS, FM, FLISA Polyclonal IgG FITC
Catalog No. ABIN100791
  • Target See all IgA, IgG, IgM products
    IgA, IgG, IgM
    Binding Specificity
    • 74
    • 58
    • 31
    • 16
    • 9
    Heavy & Light Chain
    Reactivity
    • 71
    • 47
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Human
    Host
    • 160
    • 55
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 218
    • 1
    Polyclonal
    Conjugate
    • 54
    • 38
    • 33
    • 27
    • 22
    • 11
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    FITC
    Application
    • 117
    • 112
    • 77
    • 73
    • 67
    • 56
    • 26
    • 23
    • 22
    • 22
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
    Specificity
    IgG IgA IgM
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using antigens coupled to agarose beads
    Immunogen

    Immunogen: Human IgG, IgA and IgM whole molecules

    Isotype
    IgG
    Labeling Ratio
    4.9
  • Application Notes

    Application Note: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgA, IgG, IgM
    Alternative Name
    IgG IgA IgM (IgA, IgG, IgM Products)
    Target Type
    Antibody
    Background

    Synonyms: Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgG IgA IgM Antibody fluorescein Conjugated, Goat Anti-Human IgGAM Antibody FITC Conjugated

    Background: Anti-Human IgG IgA IgM (H&L) Fluorescein Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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