Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN100792

Product Details

Target: IgA, IgG, IgM

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB)

Specificity: IgG IgA IgM

Characteristics: Anti-Human IgG IgA IgM (H&L) antibody generated in goat detects specifically human IgG IgA IgM (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.

Isotype: IgG

Application Details

Application Notes: Anti-Human IgG IgA IgM (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

Crawford, Tempel, Streblow, Kreklywich, Smith, Picker, Nelson, Caposio: "Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice." in: Scientific reports, Vol. 7, Issue 1, pp. 937, (2018) (PubMed).

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM (IgA, IgG, IgM Products)

Background: Synonyms: Human Antibody, Gt-a-Human IgG, Human Antibody in Goat, Human Secondary Antibody