Goat anti-Mouse (Murine) IgA, IgG, IgM (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Details for Product No. ABIN100806
Antigen
Epitope
Heavy & Light Chain
65
35
9
5
5
Reactivity
Mouse (Murine)
61
32
20
15
11
9
4
4
3
3
3
2
2
1
1
Host
Goat
106
56
4
3
2
Clonality
Polyclonal
Conjugate
FITC
28
26
18
14
9
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogen

Immunogen: Mouse IgG IgA and IgM whole molecule

Isotype IgG
Specificity IgG IgA IgM
Cross-Reactivity Mouse (Murine)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using antigens coupled to agarose beads
Labeling Ratio 2.99
Antigen
Alternative Name IgG IgA IgM
Background

Synonyms: Goat anti-mouse IgG IgA IgM antibody fluorescein conjugation, goat anti-mouse IgGAM FITC conjugated antibody

Background: Anti-Mouse IgG IgA IgM Fluorescein Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Mouse (Murine) IgA, IgG, IgM (Heavy & Light Chain) antibody (FITC) - Preadsorbed (ABIN100806) Dot Blot for Goat Anti-Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Dot Bl...