Goat anti-Mouse IgA, IgG, IgM Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB
Polyclonal
IgG
Alkaline Phosphatase (AP)
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 610-105-430
- Supplier
- Rockland
- Purpose
- Mouse IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- Cross-Reactivity (Details)
- Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all Mouse immunoglobulin isotypes, subclasses and chain combinations.
- Characteristics
- Anti-Mouse IgG IgA IgM Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Mouse IgG IgA IgM Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Expiry Date
- 12 months
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG,IgA,IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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