Goat anti-Mouse (Murine) IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Details for Product No. ABIN100814
Antigen
Epitope
Heavy & Light Chain
65
35
9
5
5
Reactivity
Mouse (Murine)
61
32
20
15
11
9
4
4
3
3
3
2
2
1
1
Host
Goat
106
56
4
3
2
Clonality
Polyclonal
Conjugate
HRP
27
27
18
14
9
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Mouse IgG IgA and IgM whole molecule

Isotype IgG
Specificity IgG IgA IgM
Cross-Reactivity Mouse (Murine)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Antigen
Alternative Name IgG IgA IgM
Background

Synonyms: Goat anti-mouse IgG IgA IgM antibody peroxidase conjugation, goat anti-mouse IgGAM HRP conjugated antibody

Background: Anti-Mouse IgG IgA IgM Peroxidase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Mouse IgG IgA IgM Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Mouse (Murine) IgA, IgG, IgM (Heavy & Light Chain) antibody (HRP) - Preadsorbed (ABIN100814) ELISA results of purified Goat Anti-MOUSE IgG IgA IgM Peroxidase Antibody (Min X Bv, ...