Rabbit anti-Cow (Bovine) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN100896
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Cow (Bovine)
355
342
336
233
232
122
113
87
80
72
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Bovine IgG whole molecule

Specificity IgG (H&L)
Characteristics Concentration Definition: by Refractometry
Purification This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Bovine IgG and Bovine Serum.
Antigen
Background

Synonyms: rabbit anti-Bovine IgG Antibody, rabbit anti Bovine IgG

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Bovine IgG antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 27.0 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative Without preservative
Storage RT,4 °C,-20 °C
Expiry Date 12 months