Goat anti-Chicken IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed Secondary Antibody
- Binding Specificity
Heavy & Light Chain
- FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- IgG (H&L)
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
Immunogen: Chicken IgG whole molecule
- Labeling Ratio
- Application Notes
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- For Research Use only
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 2.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
Synonyms: goat anti-Chicken IgG Rhodaime Conjugated Antibody, goat anti-Chicken IgG Antibody TRITC Conjugation, Chicken Secondary Antibody
Background: Anti-Chicken IgG Rhodamine Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.