Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Goat anti-Chicken IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG TRITC
Catalog No. ABIN101012
$240.00
Plus shipping costs $45.00
2 mg
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    IgG
    Binding Specificity
    Heavy & Light Chain
    • 1530
    • 134
    • 117
    • 53
    • 34
    • 30
    • 25
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Reactivity
    Chicken
    • 367
    • 335
    • 315
    • 224
    • 214
    • 111
    • 110
    • 82
    • 75
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Goat
    • 829
    • 781
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1795
    • 177
    Conjugate
    TRITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificity
    IgG (H&L)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
    Immunogen

    Immunogen: Chicken IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    3.11
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Background

    Synonyms: goat anti-Chicken IgG Rhodaime Conjugated Antibody, goat anti-Chicken IgG Antibody TRITC Conjugation, Chicken Secondary Antibody

    Background: Anti-Chicken IgG Rhodamine Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

You are here:
help Support