Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 μg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:46,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:10,000