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Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody (HRP)

ELISA, IHC, WB Polyclonal IgG HRP
Rockland
Catalog No. ABIN101039
Supplier Product No.: 203-4302
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2397
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 687
    • 677
    • 573
    • 433
    • 381
    • 208
    • 195
    • 144
    • 127
    • 125
    • 107
    • 102
    • 96
    • 88
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Chicken
    Host
    • 1706
    • 1430
    • 525
    • 205
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3130
    • 172
    • 16
    • 1
    Polyclonal
    Conjugate
    • 587
    • 415
    • 415
    • 367
    • 274
    • 262
    • 235
    • 118
    • 85
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 44
    • 39
    • 39
    • 37
    • 35
    • 35
    • 32
    • 31
    • 31
    • 30
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 23
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    • 15
    • 15
    • 15
    • 15
    • 13
    • 10
    • 8
    HRP
    Application
    • 2038
    • 1984
    • 1823
    • 1778
    • 998
    • 920
    • 896
    • 487
    • 424
    • 149
    • 98
    • 64
    • 56
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    203-4302
    Supplier
    Rockland
    Purpose
    Chicken IgG (H&L) Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
    Characteristics
    Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen
    Optional[Immunogen]: Chicken IgG whole molecule
    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Chicken IgG Peroxidase Antibody has been tested by ELISA. This product has been assayed against 1.0 μg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:46,000 of the reconstitution concentration is suggested for this product. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:None
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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