Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (Biotin)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Goat
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 605-706-002
- Supplier
- Rockland
- Purpose
- Goat IgG (H&L) Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Goat IgG and Goat Serum.
- Characteristics
- Anti-Goat IgG Alkaline Phosphatase Antibody generated in donkey detects goat IgG.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
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Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Goat IgG Biotin Conjugated Antibody has been tested by ELISA and Dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:200,000 - 1:250,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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