Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed

Catalog No. ABIN1043943

Supplier Product No.: 605-453-013s

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Atto 532

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 605-453-013s

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody ATTO 532 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Hu, Ms, Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.

Characteristics: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG.

Purification: Goat IgG (H&L) conjugated to ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Goat IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Goat IgG (H&L) conjugated to ATTO 532 has been tested by western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Sarmento, Oneto, Pelicci, Pesce, Scipioni, Faretta, Furia, Dellino, Pelicci, Bianchini, Diaspro, Lanzanò: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, (2018) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.