Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed Secondary Antibody

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Catalog No. ABIN101295
$316.50
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    IgG
    Binding Specificity
    Heavy & Light Chain
    • 1530
    • 134
    • 117
    • 53
    • 34
    • 30
    • 25
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Reactivity
    Guinea Pig
    • 367
    • 335
    • 315
    • 224
    • 214
    • 111
    • 109
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Rabbit
    • 830
    • 780
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1795
    • 177
    Conjugate
    Alkaline Phosphatase (AP)
    Application
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG (H&L)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Guinea Pig IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-GUINEA PIG IgG (H&L) (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.

    ELISA Dilution: 1:5,000 - 1:15,000

    Western Blot Dilution: 1:500 - 1:2,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    IgG
    Background

    Synonyms: rabbit Anti-Guinea Pig IgG Antibody alkaline phosphatase Conjugation, rabbit Anti-Guinea Pig IgG alk phos Conjugated antibody

    Background: Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in rabbit detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

You are here:
help Support