Rabbit anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed Secondary Antibody
- Binding Specificity
Heavy & Light Chain
- Alkaline Phosphatase (AP)
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- IgG (H&L)
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
- Sterile filtered
Immunogen: Guinea Pig IgG whole molecule
- Application Notes
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-GUINEA PIG IgG (H&L) (RABBIT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.
ELISA Dilution: 1:5,000 - 1:15,000
Western Blot Dilution: 1:500 - 1:2,000
- For Research Use only
- 1.0 mg/mL
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
- 4 °C
- Expiry Date
- 12 months
Synonyms: rabbit Anti-Guinea Pig IgG Antibody alkaline phosphatase Conjugation, rabbit Anti-Guinea Pig IgG alk phos Conjugated antibody
Background: Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in rabbit detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.