Goat anti-Goat IgG (Fc Region) Antibody - Preadsorbed

Details for Product No. ABIN101444
Antigen
Epitope
Fc Region
1570
134
99
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Goat, Horse (Equine)
355
342
336
233
231
122
113
87
80
73
72
64
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Options
Immunogen

Immunogen: Horse IgG F(c) fragment

Isotype IgG
Specificity IgG F(c)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using Horse IgG coupled to agarose beads
Sterility Sterile filtered
Antigen
Background

Synonyms: goat Anti-Horse IgG F(c) Antibody, goat Anti-Horse IgG Fc Fragment Antibody

Background: Horse IgG F(c) antibody generated in goat recognizes the F(c) portion of horse IgG. It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Horse IgG F(c) antibody is ideal for investigators involved in serum component protein research.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Horse IgG F(c) antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Liquid
Concentration 2.1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months