Goat anti-Goat IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Details for Product No. ABIN101511
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Goat, Human
355
341
336
233
231
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
Alkaline Phosphatase (AP)
236
220
209
149
147
130
75
49
48
35
31
26
26
26
26
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Options
Immunogen

Immunogen: Human IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Human IgG (H&L) detects human Immunoglobulin G (IgG) antibody isotype.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using Human IgG coupled to agarose beads
Sterility Sterile filtered
Antigen
Background

Synonyms: goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody

Background: Anti-Human IgG (H&L) Alkaline Phosphatase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Human IgG (H&L) Alkaline Phosphatase Antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Antibody should be optimized by end user for specific reactive conditions.

ELISA Dilution: 1:4,500

Western Blot Dilution: 1:500 - 1:2,500

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN101511) Western Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Lane 1:...
Image no. 2 for Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN101511) Dot Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Antigen: Hu...