Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed Secondary Antibody
- Binding Specificity
- Heavy & Light Chain
- Alkaline Phosphatase (AP)
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- IgG (H&L)
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
- Sterile filtered
Immunogen: Human IgG whole molecule
- Application Notes
Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Human IgG Alkaline Phosphatase Antibody is suitable for ELISA, Western Blot and immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- For Research Use only
- 1.0 mg/mL
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
- 4 °C
- Expiry Date
- 12 months
Synonyms: rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugation, rabbit-anti-Human IgG Alkaline Phosphatase Conjugated Antibody
Background: Anti-Human IgG Alkaline Phosphatase Antibody generated in rabbit detects human Immunoglobulin G (IgG). It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Human IgG antibody detects human IgG whole molecule. Anti-Human IgG antibody is ideal for investigators involved in serum protein component research.