Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed Secondary Antibody
- Binding Specificity
- Heavy & Light Chain
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- IgG (H&L)
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
Immunogen: Human IgG whole molecule
- Application Notes
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:220,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- For Research Use only
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 2.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
Synonyms: Rabbit Anti Human IgG Biotin Conjugated Antibody, Rabbit Anti-Human IgG Antibody Biotin Conjugation
Background: Anti-Human IgG (H&L) Biotin generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.