Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed Secondary Antibody
- Binding Specificity
- Heavy & Light Chain
- FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- IgG (H&L)
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
Immunogen: Human IgG whole molecule
- Labeling Ratio
- Application Notes
Application Note: Anti-Human IgG (H&L) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
Excitation/Emission wavelength: 494 nm/514 nm
- For Research Use only
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 2.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
Messner, Frotschnig, Steinacher-Nigisch, Winter, Eichmair, Gebetsberger, Schwaiger, Ploner, Laufer, Bernhard: "Apoptosis and necrosis: two different outcomes of cigarette smoke condensate-induced endothelial cell death." in: Cell death & disease, Vol. 3, pp. e424, 2013 (PubMed).
- Messner, Frotschnig, Steinacher-Nigisch, Winter, Eichmair, Gebetsberger, Schwaiger, Ploner, Laufer, Bernhard: "Apoptosis and necrosis: two different outcomes of cigarette smoke condensate-induced endothelial cell death." in: Cell death & disease, Vol. 3, pp. e424, 2013 (PubMed).
Synonyms: rabbit anti-Human IgG Antibody Fluorescein Conjugation, rabbit anti-Human IgG FITC Conjugated Antibody
Background: Anti-Human IgG (H&L) Fluorescein generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.