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Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG
Pubmed (2)
Catalog No. ABIN101567
$307.50
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2 mg
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  • Target
    Binding Specificity
    Heavy & Light Chain
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Texas Red (TR)
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificity
    IgG (H&L)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Human IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.0
  • Application Notes

    Application Note: Anti-Human IgG (H&L) Texas Red is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Texas Red™ is a registered trademark of Molecular Probes Inc.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Zschüntzsch, Zhang, Klinker, Makosch, Klinge, Malzahn, Brinkmeier, Liebetanz, Schmidt: "Treatment with human immunoglobulin G improves the early disease course in a mouse model of Duchenne muscular dystrophy." in: Journal of neurochemistry, Vol. 136, Issue 2, pp. 351-62, 2016 (PubMed).

    Zschüntzsch, Voss, Creus, Sehmisch, Raju, Dalakas, Schmidt: "Provision of an explanation for the inefficacy of immunotherapy in sIBM: Quantitative assessment of inflammation and β-amyloid in the muscle." in: Arthritis and rheumatism, 2012 (PubMed).

  • Target
    Background

    Synonyms: Rabbit Anti Human IgG Texas Red™ Conjugated Antibody, Rabbit Anti-Human IgG Antibody Texas Red™ Conjugation

    Background: Anti-Human IgG (H&L) Texas Red generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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