Rabbit anti-Human IgG (Fc Region) Antibody Secondary Antibody
- Binding Specificity
- Fc Region
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- IgG F(c)
- Concentration Definition: by Refractometry
- Human IgG F(c) Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
Immunogen: Human IgG F(c) fragment
- Application Notes
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- For Research Use only
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 80.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
Synonyms: rabbit anti-Human IgG F(c) fragment Antibody, rabbit anti-Human IgG Fc fragment Antibody, rabbit anti Human IgG Fc
Background: Anti-Human IgG F(c) generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.