Rabbit anti-Human IgG (Fc Region) Antibody - Preadsorbed
- Target See all IgG products
- Fc Region
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- IgG F(c)
Anti-Human IgG F(c) fragment antibody generated in rabbit detects specifically Human IgG F(c) fragment. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
- Sterile filtered
Immunogen: Anti-Human IgG F(c) fragment was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
Immunogen Type: Native Protein
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- Application Notes
Immunohistochemistry Dilution: 1:1,000 - 1:6,000
Application Note: Anti-Human IgG F(c) fragment is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA Dilution: 1:375,000
Western Blot Dilution: 1:3,000 - 1:30,000
- For Research Use only
- 4.8 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative: 0.01 % (w/v) Sodium Azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C,-20 °C
- Expiry Date
- 12 months
- IgG Products
- Target Type
Synonyms: Human IgG F(c) Antibody, Human IgG Fc Antibody, Rb-a-Human IgG Fc, Human IgG F(c) Antibody in Rabbit, rabbit anti-human IgG F(c) Secondary Antibody
Background: Anti-Human IgG F(c) generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.