Goat anti-Rabbit IgG (Fc Region) Antibody - Preadsorbed Secondary Antibody
- Fc Region
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Anti-Rabbit IgG F(c) antibody generated in goat detects specifically rabbit IgG F(c). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Synonyms: Rabbit Antibody F(c), Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Rabbit F(c) Secondary Antibody
- Preadsorption: Solid phase absorption
- Sterile filtered
Immunogen: Anti Rabbit IgG F(c) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
Immunogen Type: Native Protein
- Application Notes
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Rabbit IgG F(c) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
Concentration (detection method): 2.0 mg/mL (by UV absorbance at 280 nm)
- For Research Use only
- 2.0 mg/mL
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C,-20 °C
- Expiry Date
- 12 months
Synonyms: goat anti-Rabbit F(c) Antibody, Gt-a-Rabbit IgG F(c), Rabbit Antibody in Goat, Goat anti-Rabbit F(c) fragment Secondary Antibody
Background: Anti-Rabbit IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.