Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN102218
Antigen
Epitope
Heavy & Light Chain
1572
134
101
57
34
33
25
6
6
6
6
5
4
4
4
3
3
1
1
1
Reactivity
Sheep (Ovine)
356
343
337
234
233
122
113
87
80
76
72
67
61
57
55
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Donkey
847
800
337
169
93
85
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Sheep IgG whole molecule

Immunogen Type: Native Protein

Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Sheep IgG unconjugated antibody was generated in donkey and detects specifically sheep IgG. This primary unconjugated anti-Sheep antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Purification Sheep IgG (H&L) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. This antibody will detect intact and immunoglobulin components from Sheep.
Antigen
Background

Synonyms: donkey anti-Sheep IgG Antibody, donkey anti-Sheep IgG Antibody

Background: Anti-Sheep IgG unconjugated antibody was generated in donkey and detects specifically sheep IgG. This primary unconjugated anti-Sheep antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Sheep IgG unconjugated antibody is suitable for multiple immunoassays including immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The antibody may be conjugated to the end user specifications.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 5.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 10.0 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) antibody (ABIN102218) Western Blot of Donkey anti-Sheep IgG Antibody. Lane 1: Sheep IgG. Lane 2: None. Load...