Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Details for Product No. ABIN102222
Antigen
Epitope
Heavy & Light Chain
1571
134
101
57
34
33
25
6
6
6
6
5
4
4
4
3
3
1
1
1
Reactivity
Sheep (Ovine)
356
343
337
234
233
122
112
87
80
76
72
67
61
57
55
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Donkey
847
800
336
169
93
85
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
Biotin
238
220
208
150
147
130
75
50
48
36
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Anti-Sheep IgG whole molecule was produced by repeated immunization with Sheep IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype IgG
Specificity IgG (H&L)
Cross-Reactivity Sheep (Ovine)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Antigen
Background

Synonyms: Donkey Anti-Sheep IgG biotin Conjugated Antibody, Donkey Anti-Sheep IgG Antibody BAC Conjugation

Background: Sheep IgG antibody generated in donkey detects specifically Sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Sheep IgG is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Aliquot to Avoid repeated freezing and thawing.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed (ABIN102222) ELISA results of purified Donkey anti-Sheep IgG Biotin Conjugated Antibody tested aga...
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).