Rabbit anti-Sheep IgG (F(ab')2 Region) Antibody (FITC) - Preadsorbed Secondary Antibody
- Binding Specificity
- F(ab')2 Region
- FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- IgG F(ab')2
- Concentration Definition: by UV absorbance at 280 nm
- Preadsorption: Solid phase absorption
Immunogen: Sheep IgG F(ab')2 fragment
- Labeling Ratio
Synonyms: Rabbit Anti-Sheep IgG F(ab')2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody FITC Conjugation
Background: Anti-Sheep IgG F(ab')2 Fluorescein Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 Antibody is conjugated to Fluorescein.
- Application Notes
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
Excitation/Emission wavelength: 494 nm/514 nm
- For Research Use only
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 1.5 mg/mL
Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- RT,4 °C,-20 °C
- Expiry Date
- 12 months