KLRK1 antibody (APC)

Catalog No. ABIN1027687

Product Details anti-KLRK1 Antibody

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLRK1 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Specificity: The mouse monoclonal antibody 1D11 recognizes CD314 / NKG2D, a 42 kDa C-type lectin-like activating receptor expressed by NK cells, gamma/delta T cells, and CD8+ T cells.

Cross-Reactivity (Details): Human

Immunogen: NKL cell line

Clone: 1D11

Isotype: IgG1

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-KLRK1 antibody (ABIN1027687)

Valencia, Hernández-López, Martínez, Hidalgo, Zapata, Vicente, Varas, Sacedón: "Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors." in: Journal of leukocyte biology, Vol. 87, Issue 3, pp. 405-14, (2010) (PubMed).

Hasenkamp, Borgerding, Uhrberg, Falk, Chapuy, Wulf, Jung, Trümper, Glass: "Self-tolerance of human natural killer cells lacking self-HLA-specific inhibitory receptors." in: Scandinavian journal of immunology, Vol. 67, Issue 3, pp. 218-29, (2008) (PubMed).

Sangiolo, Martinuzzi, Todorovic, Vitaggio, Vallario, Jordaney, Carnevale-Schianca, Capaldi, Geuna, Casorzo, Nash, Aglietta, Cignetti: "Alloreactivity and anti-tumor activity segregate within two distinct subsets of cytokine-induced killer (CIK) cells: implications for their infusion across major HLA barriers." in: International immunology, Vol. 20, Issue 7, pp. 841-8, (2008) (PubMed).

Ebert, Meuter, Moser: "Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 7, pp. 4331-6, (2006) (PubMed).

Wu, Groh, Spies: "T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial gamma delta T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1236-40, (2002) (PubMed).

Wu, Cherwinski, Spies, Phillips, Lanier: "DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells." in: The Journal of experimental medicine, Vol. 192, Issue 7, pp. 1059-68, (2000) (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, (1999) (PubMed).

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: CD314 (KLRK1 Products)

Synonyms: KLRK1 antibody, NKG2D antibody, CD314 antibody, D12S2489E antibody, KLR antibody, NKG2-D antibody, D6H12S2489E antibody, Nkg2d antibody, Nkrp2 antibody, killer cell lectin like receptor K1 antibody, killer cell lectin-like receptor subfamily K, member 1 antibody, NKG2D protein antibody, KLRK1 antibody, Klrk1 antibody, NKG2D antibody

Background: CD314, also known as NKG2D (natural killer receptor G2D) or KLRK1 (killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process