Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (Atto 532) - Preadsorbed

Details for Product No. ABIN1043943
Antigen
Epitope
Heavy & Light Chain
1566
134
100
55
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Goat
355
341
336
232
231
121
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
8
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
842
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
Atto 532
236
219
209
149
147
130
75
49
48
35
31
26
26
26
25
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
15
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Goat IgG whole molecule

Isotype IgG
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Characteristics Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Preadsorption: Solid phase absorption
Labeling Ratio 3.2
Antigen
Background

Synonyms: rabbit anti-Goat IgG ATTO 532 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO532 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Notes

Application Note: Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Expiry Date 12 months
Supplier Images
Image no. 1 for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed (ABIN1043943) Western Blot of Atto 532 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. ...
Image no. 2 for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed (ABIN1043943) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - Preadsorbed (Image 2)
Product cited in: Sarmento, Oneto, Pelicci, Pesce, Scipioni, Faretta, Furia, Dellino, Pelicci, Bianchini, Diaspro, Lanzanò: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, 2018 (PubMed).