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Donkey anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG
Catalog No. ABIN1043950
$333.00
Plus shipping costs $45.00
1 mg
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  • Target
    Binding Specificity
    Heavy & Light Chain
    Reactivity
    Guinea Pig
    Host
    Donkey
    Clonality
    Polyclonal
    Conjugate
    TRITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum.
    Cross-Reactivity
    Guinea Pig
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads
    Immunogen

    Immunogen: Guinea Pig IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.4
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Centrifuge product if not completely clear after standing at room temperature.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    Background

    Synonyms: Donkey Anti-Guinea Pig IgG Rhodamine Conjugation, Donkey Anti-Guinea Pig IgG TRITC Conjugated

    Background: Anti-Guinea Pig IgG Rhodamine Antibody generated in donkey detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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