Mouse anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
- Target See all IgG products
- Heavy & Light Chain
- Alkaline Phosphatase (AP)
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
- Preadsorption: Solid phase absorption
- Sterile filtered
Immunogen: Human IgG whole molecule
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- Application Notes
Immunohistochemistry Dilution: 1:200-1:1,000
Application Note: Anti-Human IgG Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000-1:10,000
Western Blot Dilution: 1:500-1:2,500
- For Research Use only
- 1 mg/mL
Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature.
- 4 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Expiry Date
- 12 months
- IgG Products
- Target Type
Synonyms: mouse anti-Human IgG alkaline phosphatase conjugated Antibody, mouse anti-Human IgG Antibody alk phos conjugation
Background: Anti-Human IgG (H&L) Alkaline Phosphatase generated in mouse detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.