GPR50 antibody (G Protein-Coupled Receptor 50) (C-Term)

Details for Product anti-GPR50 Antibody No. ABIN1049058
Antigen
  • H9
  • Mel1c
  • G protein-coupled receptor 50
  • G-protein-coupled receptor 50
  • GPR50
  • Gpr50
Epitope
C-Term
10
9
9
9
8
7
4
2
2
1
1
1
Reactivity
Human
64
12
8
3
2
2
1
1
Host
Rabbit
59
5
Clonality
Polyclonal
Conjugate
This GPR50 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
40
23
22
19
9
7
3
1
Options
Brand IHC-plus™
Immunogen Synthetic 20 amino acid peptide from C-terminus of human GPR50. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey (95%), Marmoset (90%), Gibbon (85%).

Type of Immunogen: Synthetic peptide
Specificity Human GPR50. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (95%) Marmoset (90%) Gibbon (85%).
Purification Immunoaffinity purified
Plasmids, Primers & others Plasmids, Primers & others GPR50 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name GPR50 (GPR50 Antibody Abstract)
Background Name/Gene ID: GPR50
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR50, G protein-coupled receptor 50, Mel1c, Melatonin-related receptor, MTNRL, H9
Gene ID 9248
Application Notes Approved: IHC, IHC-P (3 - 28 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-G Protein-Coupled Receptor 50 (GPR50) (C-Term) antibody (ABIN1049058) Anti-GPR50 antibody ABIN1049058 IHC staining of human pituitary. Immunohistochemistr...
Immunohistochemistry (IHC) image for anti-G Protein-Coupled Receptor 50 (GPR50) (C-Term) antibody (ABIN1049058) Anti-GPR50 antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, p...
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