Papain antibody

Catalog No. ABIN105334

Supplier Product No.: 200-1174-0100

Product Details

Target: Papain

Reactivity: Papaya

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 200-1174-0100

Supplier: Rockland

Purpose: Papain Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

Characteristics: Synonyms: goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

Purification: Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: Anti-Papain antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Papain

Background: Background: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

UniProt: P00784