CNF1 (AA 704-730) antibody

Catalog No. ABIN1106727

Product Details

Target: CNF1

Binding Specificity: AA 704-730

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Dot Blot (DB), Enzyme Immunoassay (EIA)

Specificity: The Monoclonal antibody clone NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.

Immunogen: Synthetic peptide, corresponding to amino acids 704/730 of Escherichia coli CNF1

Clone: NG8

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 % BSA

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: CNF1

Background: CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.Synonyms: Cytotoxic necrotizing factor 1

Molecular Weight: 115 kDa

Gene ID: 3990770

NCBI Accession: YP_543855

UniProt: Q1R2U0