BACH2 antibody (N-Term)

Catalog No. ABIN1118465

Supplier Product No.: 600-401-h35

Product Details anti-BACH2 Antibody

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Binding Specificity: N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-h35

Supplier: Rockland

Purpose: Bach2 Antibody

Cross-Reactivity (Details): Bach2 antibody is directed against mouse Bach2 (n-terminus) protein.

Characteristics: Synonyms: rabbit anti-Bach2 Antibody, Bach-2, Bach 2, BTB and CNC homology 1, basic leucine zipper transcription factor 2

Purification: Anti-Bach-2 was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Affinity purified Bach-2 antibody has been tested for use in ELISA, Immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91-140 kDa in size corresponding to Bach2 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

Immunoprecipitation Dilution: 1 μg/mL

ELISA Dilution: 1:5000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.97 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BACH2

Target: BACH2 (BTB and CNC Homology 1, Basic Leucine Zipper Transcription Factor 2 (BACH2))

Alternative Name: Bach2 (BACH2 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer and DNA Damage & Repair research. During an immune response to infectious diseases and cancer, B-cells undergo activation in response to antigen, and differentiate either into short-lived plasma cells, whose numbers contract following withdrawal of the antigenic stimulus, or into memory B-cells. The formation of B-cell memory is the major goal of vaccination, since memory B-cells are able to produce antibodies specific to infections and cancer that can last for the lifetime of the host. Recently it has been shown that the formation of long-lived memory B-cells is dependent upon the transcription factor Bach2, since knockout mice lacking the gene fail to generate class-switched IgG antibody responses and all B-cells undergo default plasma cell differentiation upon antigenic stimulation. While the mechanism by which Bach2 controls B-cell memory fate has been elucidated (it binds and represses the promoter of the gene that encodes the plasma cell master regulatory transcription factor Blimp-1), the upstream mechanism by which the function of Bach2 is regulated is unknown. There is evidence suggesting that Bach2 is phosphorylated in B-cells following stimulation and suspect that this modification may allow the B-cell signaling apparatus to control Bach2 activity and therefore memory fate decisions. Anti-Bach2 is ideal for researchers interested in Cancer and DNA Damage & Repair research.

Gene ID: 12014

UniProt: A2RRI0