SLC25A4 antibody (N-Term)

Catalog No. ABIN112625

Product Details anti-SLC25A4 Antibody

Target: SLC25A4 (Solute Carrier Family 25 (Mitochondrial Carrier, Adenine Nucleotide Translocator), Member 4 (SLC25A4))

Binding Specificity: AA 1-11, N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-G-D-Q-A-L-S-F-L-K-D-F

Specificity: Reacts with Adenine Nucleotide Translocator 1 (ANT1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rabbit, Bovine and Chicken:Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G.

Immunogen: Synthetic peptide corresponding to the amino acids 1-11 of the native molecule Rat ANT1 conjugated to KLH

Application Details

Application Notes: ELISA: 1/500-1/2000. Western blot: Gives A peptide blockable duplet at 31/33 kD in rat heart mitochondria. Alsopart peptide blockable duplets at 38/48 and 66/70 kD.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0 mg/mL (by OD280)

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SLC25A4

Target: SLC25A4 (Solute Carrier Family 25 (Mitochondrial Carrier, Adenine Nucleotide Translocator), Member 4 (SLC25A4))

Alternative Name: SLC25A4 / ANT1 (SLC25A4 Products)

Background: Adenine Nucleotide Translocase 1 (ANT1) catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane. It is is a key component of the mitochondrial permeability transition pore (PT pore) complex and is also one of the most abundant proteins in the mitochondrion making up approximately 10 % of all mitochondrial proteins. Originally described as an ADP/ATP exchange factor, ANT1 has also been shown to be required for bax mediated apoptosis. This is effected by binding of bax to residues 105-156 of ANT1, presumably resulting in maintaining the PT pore in an open conformation. Interaction with bax is not required for ANT1 pro-apoptotic activity as overexpression of the ANT1 protein also leads to the induction of apoptosis.Synonyms: ADP/ATP translocase 1, ANT 1, Adenine nucleotide translocator 1, Solute carrier family 25 member 4

Gene ID: 85333

NCBI Accession: NP_445967

UniProt: Q05962

Pathways: Proton Transport, Dicarboxylic Acid Transport