Digoxigenin antibody
DIG
Reactivity: Digitalis purpurea (Common foxglove), Digitalis spec.
WB, IHC (fro), EIA
Host: Sheep
Polyclonal
unconjugated
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- Target See all Digoxigenin (DIG) products
- Digoxigenin (DIG)
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Reactivity
- Digitalis purpurea (Common foxglove), Digitalis spec.
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Digoxigenin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Purification
- Affinity Chromatography on Protein G .
- Immunogen
- Digoxin-3-CMO conjugated to KLH
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- Application Notes
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Immunohistochemistry on Frozen Tissue. Western blot. ELISA (1/100-1/5,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 5.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Digoxigenin (DIG)
- Alternative Name
- Digoxigenin (DIG Products)
- Target Type
- Chemical
- Background
- Digoxigenin is a steroid found in plants that is often attached to oligonucleotides and used as a molecular probe to detect mRNA production. Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
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