CXCL2 antibody (Biotin)

Catalog No. ABIN116265

Product Details anti-CXCL2 Antibody (Biotin)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects MIP-2.

Purification: Affinity Chromatography employing immobilized mMIP-2 matrix

Immunogen: Highly pure (>98%) recombinant Murine MIP- 2.

Application Details

Application Notes: ELISA: To detect mMIP-2 by direct ELISA (using 100 μL/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMIP-2. Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CXCL2 antibody (Biotin) (ABIN116265)

Sterzenbach, Lee, Brenneke, von Goetz, Schauer, Fox, Suerbaum, Josenhans: "Inhibitory effect of enterohepatic Helicobacter hepaticus on innate immune responses of mouse intestinal epithelial cells." in: Infection and immunity, Vol. 75, Issue 6, pp. 2717-28, (2007) (PubMed).

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 / MIP2A (CXCL2 Products)

Synonyms: CINC-2a antibody, GRO2 antibody, GROb antibody, MGSA-b antibody, MIP-2a antibody, MIP2 antibody, MIP2A antibody, SCYB2 antibody, GRO3 antibody, Gro2 antibody, MIP-2 antibody, Mgsa-b antibody, Mip2 antibody, Scyb antibody, Scyb2 antibody, Mip-2 antibody, GROB antibody, MIP-2alpha antibody, C-X-C motif chemokine ligand 2 antibody, chemokine (C-X-C motif) ligand 2 antibody, CXCL2 antibody, Cxcl2 antibody

Background: GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

Gene ID: 20310

NCBI Accession: NP_033166

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin