Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CXCL2 antibody (Chemokine (C-X-C Motif) Ligand 2) (Biotin) Primary Antibody

CXCL2 Reactivity: Mouse EIA, WB Host: Rabbit Polyclonal Biotin
Pubmed (1)
Catalog No. ABIN116265
$399.30
Plus shipping costs $45.00
25 μg
local_shipping Shipping to: United States
Available
  • Target
    CXCL2
    Reactivity
    • 40
    • 19
    • 18
    • 2
    Mouse
    Host
    • 66
    • 7
    • 3
    • 1
    Rabbit
    Clonality
    • 72
    • 5
    Polyclonal
    Conjugate
    • 36
    • 13
    • 7
    • 6
    • 5
    • 5
    • 5
    This CXCL2 antibody is conjugated to Biotin
    Application
    • 70
    • 59
    • 27
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    Enzyme Immunoassay (EIA), Western Blotting (WB)
    Specificity
    This antibody detects MIP-2.
    Purification
    Affinity Chromatography employing immobilized mMIP-2 matrix
    Immunogen
    Highly pure (>98%) recombinant Murine MIP- 2.
  • Application Notes
    ELISA: To detect mMIP-2 by direct ELISA (using 100 μL/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMIP-2. Western Blot: To detect mMIP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mMIP-2 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Sterzenbach, Lee, Brenneke, von Goetz, Schauer, Fox, Suerbaum, Josenhans: "Inhibitory effect of enterohepatic Helicobacter hepaticus on innate immune responses of mouse intestinal epithelial cells." in: Infection and immunity, Vol. 75, Issue 6, pp. 2717-28, 2007 (PubMed).

  • Target
    CXCL2
    Alternative Name
    CXCL2 / MIP2A (CXCL2 Antibody Abstract)
    Synonyms
    CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, MIP2, MIP2A, SCYB2, GRO3, Gro2, MIP-2, Mgsa-b, Mip2, Scyb, Scyb2, Mip-2, GROB, MIP-2alpha, C-X-C motif chemokine ligand 2, chemokine (C-X-C motif) ligand 2, CXCL2, Cxcl2
    Background
    GRO beta is a member of the CXC, or chemokine class. It contains the ELR domain immediately preceding the first cysteine residue near the amino terminus. Other chemokines in this group include IL8, GRO alpha/beta/gamma, mouse KC, ENA78, GCP2, PBP/CTAPIII/beta TG/NAP2. These chemokines act primarily on neutrophils as chemoattractants and activators, including neutrophil degradation with release of myloperoxidase and other enzymes. GRO beta was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. GRO beta is an approximately 8 kDa polypeptide of 73 amino acids. The precursor form of GRO beta consists of 100 amino acids. To generate the mature GRO beta, the precursor cleaves its amino terminal 27 amino acids. GRO beta shows 60 % amino acid homology to human GRO alpha and GRO gamma.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2
    Gene ID
    20310
    NCBI Accession
    NP_033166
    UniProt
    P10889
    Pathways
    Cellular Response to Molecule of Bacterial Origin
You are here:
help Support