Sirolimus antibody
Reactivity: Various Species
AP, ELISA, IA
Host: Sheep
Polyclonal
unconjugated
-
- Target
- Sirolimus
- Reactivity
- Various Species
- Host
-
Sheep
- Clonality
- Polyclonal
- Application
- Affinity Purification (AP), ELISA, Immunoassay (IA)
- Purpose
- Sirolimus
- Sensitivity
-
10ng/mL
Prednisolone produces 59% inhibition in a competitive ELISA employing Prednisolone polyclonal Antibody - Purification
- Salt fractionation.
- Immunogen
- Sirolimus-BTG
- Isotype
- IgG
-
- Application Notes
-
Application_Dilutions: 2.5 μg/mL
Tested_Applications: The antibody is suitable for the development of immunoassays or immunoaffinity purification columns.
- Comment
-
10 ng/mL Rapamycin (Sirolimus) produces 63 % inhibition in a competitive ELISA employing Sirolimus polyclonal antibody.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 20 mM Phosphate, 150 mM Sodium Chloride. pH 7.4, 0.09 % Sodium Azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Can be stored for up to 3 months at +2°C - +8°C. For long term storage, aliquot and store at ≤-20 °C. Avoid repeated freeze/thaw cycles. Product should be protected from light exposure.
-
- Target
- Sirolimus
- Target Type
- Chemical
- Background
- Dehydroepiandrosterone is the most abundant adrenal androgen in humans and exists predominately in a sulfated form (DHEAS) [1]. DHEA sulfotransferase, known as SULT2A1, converts the androgen precursor DHEA to its inactive sulfate ester, DHEAS. The unconjugated molecules can be converted directly to androgens. Dehydroepiandrosterone is converted to DHEAS in the adrenal glands and liver [2]. DHEA in adults is present at plasma concentrations 100 to 500 times higher than those of testosterone. DHEA can be metabolized to androgens and/or estrogens in the prostate [3]. Hepatic metabolites include 16alpha-hydroxy-DHEA, 7alpha-hydroxy-DHEA, and 7-oxo-DHEA [4]. 7beta-OH-DHEA is a metabolite [5]. The major metabolite produced in humans is a mono-hydroxylated DHEA species [4]. Synthesis of a 7alpha-hydroxy-DHEA and 5-androstene-3beta, 17beta-diol occurs in the frontal cortex, hippocampus, aμgdala, cerebellum and striatum [6].
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