Immunohistochemistry with formalin fixed paraffin embedded tissues: 11 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
For Research Use only
PBS containing 0.09 % Sodium Azide as preservative.
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
4 °C/-80 °C
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
GPR43 is classified as a Carboxylic Acid GPCR. The location of this receptor in the immune system is suggestive of potential roles in immune cell function and hematopoiesis. GPR43 has been reported primarily in immune cells including monocytes and neutrophils. An EST has been isolated from a human pancreatic library. Ligand: Short chain carboxylic acids, such as acetate.Synonyms: Free fatty acid receptor 2, G-protein coupled receptor 43, GPR43