BUBR1 Kinase Kinase (AA 1-350) antibody

Catalog No. ABIN129516

Supplier Product No.: 200-301-902

Product Details

Target: BUBR1 Kinase Kinase

Binding Specificity: AA 1-350

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Western Blotting (WB)

Supplier Product No.: 200-301-902

Supplier: Rockland

Purpose: BUBR1 Kinase Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against human BUBR1 Kinase protein.

Characteristics: Synonyms: mouse anti-BUBR1 antibody, Beta homolog of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1

Purification: The product was purified from tissue culture supernatant by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.

Immunogen Type: Recombinant Protein

Clone: 8G1

Isotype: IgG1

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:200 - 1:1,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References

Lázaro-Diéguez, Ispolatov, Müsch: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).

Target Details

Target: BUBR1 Kinase Kinase

Background: Background: BUBR1 Kinase (BUB1-related protein kinase) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores.  This protein is highly expressed in thymus followed by spleen.  Defects in BUB1B are associated with tumor formation.

Gene ID: 701

NCBI Accession: NP_001202

UniProt: O60566