SMARCAL1 antibody (AA 75-100)

Catalog No. ABIN129604

Supplier Product No.: 600-401-497

Product Details anti-SMARCAL1 Antibody

Target: SMARCAL1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1))

Binding Specificity: AA 75-100

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMARCAL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-497

Supplier: Rockland

Purpose: SmarcAL1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SmarcAL1 protein.

Characteristics: Synonyms: rabbit anti-SMARCAL1 antibody, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA Related Protein antibody, hHARP antibody, SMARCA like Protein 1 antibody, Sucrose nonfermenting protein 2 like 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal near aa 75-100 of human SmarcAL1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 106 kDa in size corresponding to SmarcAL1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:16,000 - 1:64,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMARCAL1

Target: SMARCAL1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A-Like 1 (SMARCAL1))

Alternative Name: SMARCAL1 (SMARCAL1 Products)

Background: Background: SmarcAL1 (SWI/SNF-related μatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related.  SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Gene ID: 50485, 56117838

UniProt: Q9NZC9

Pathways: Embryonic Body Morphogenesis