NCAPH antibody (AA 435-460)

Catalog No. ABIN129615

Supplier Product No.: 600-401-682

Product Details anti-NCAPH Antibody

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Binding Specificity: AA 435-460

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCAPH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-682

Supplier: Rockland

Purpose: BRRN1 Antibody

Cross-Reactivity (Details): Reactivity occurs against human BRRN1 protein.

Characteristics: Synonyms: rabbit anti-BRRN-1 Antibody, BRRN1, BRRN 1, Barren Drosophila Homolog of 1 antibody, Barren homolog protein 1 antibody, BRRN antibody, CAP H antibody, CAPH antibody, Chromosome associated protein H antibody, Condensin complex subunit 2 antibody, Condensin subunit 2 antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 435-460 of Human BRRN-1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 83 kDa in size corresponding to human BRRN1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:8,000 - 1:35,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NCAPH

Target: NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))

Alternative Name: NCAPH (NCAPH Products)

Background: Background: BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosome-associated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family.  Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.   The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG.   BRRN1 has both a nuclear and cytoplasmic localization.  In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells.  CDC2 phosphorylates BRRN1.  Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.

Gene ID: 23397

NCBI Accession: NP_001268639

UniProt: Q15003