GGA2 antibody (AA 240-270)

Catalog No. ABIN129630

Supplier Product No.: 600-401-849

Product Details anti-GGA2 Antibody

Target: GGA2 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 2 (GGA2))

Binding Specificity: AA 240-270

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-849

Supplier: Rockland

Purpose: GGA2 Antibody

Characteristics: Synonyms: rabbit anti-GGA-2 antibody, GGA2, GGA 2, Golgi associated gamma adaptin ear containing ARF binding protein 2, ADP-ribosylation factor-binding protein GGA2, Gamma-adaptin-related protein 2, VHS domain and ear domain of gamma-adaptin, Vear

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 240-270 of Human GGA2.  SwissProt accession Q9UJY4. Expected to cross-react with mouse.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GGA2

Target: GGA2 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 2 (GGA2))

Alternative Name: GGA2 (GGA2 Products)

Background: Background: GGA-2 plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. It mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain that mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

Gene ID: 23062

NCBI Accession: NP_055859

UniProt: D3DWF0