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VSV-G Epitope Tag antibody (Biotin) Primary Antibody

Reactivity: Tag FM, ELISA Host: Rabbit Polyclonal
Catalog No. ABIN129734
$610.50
Plus shipping costs $45.00
100 μg
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  • Target
    Reactivity
    Tag
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Biotin
    Application
    Fluorescence Microscopy (FM), ELISA
    Sequence
    YTDIEMNRLG K
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
    Isotype
    IgG
  • Target
    Alternative Name
    VSV-G Tag
    Target Type
    Tag
    Background
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA.  VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pHCMV-VSV-G.
  • Application Notes
    This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user.  For standard indirect immunofluor-escence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.  Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200.   At higher concentrations, a filamentous background staining may be present.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
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