CD3 epsilon antibody

Catalog No. ABIN135464

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Porcine CD3, Mr 23 kDa

Characteristics: Mouse Anti-Porcine CD3e-UNLB

Purification: Purified

Immunogen: Purified CD3 molecules from porcine thymus

Clone: PPT3

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature , Stim - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 epsilon antibody (ABIN135464)

Lei, Ryu, Wen, Twitchell, Bui, Ramesh, Weiss, Li, Samuel, Clark-Deener, Jiang, Lee, Yuan: "Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency." in: Scientific reports, Vol. 6, pp. 25222, (2018) (PubMed).

Erneholm, Lorenzen, Bøje, Olsen, Andersen, Cassidy, Follmann, Jensen, Agerholm: "Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D." in: BMC veterinary research, Vol. 12, Issue 1, pp. 200, (2017) (PubMed).

Twitchell, Tin, Wen, Zhang, Becker-Dreps, Azcarate-Peril, Vilchez, Li, Ramesh, Weiss, Lei, Bui, Yang, Schultz-Cherry, Yuan: "Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs." in: Gut pathogens, Vol. 8, pp. 51, (2016) (PubMed).

Wen, Bui, Li, Liu, Li, Kocher, Yuan: "Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection." in: Comparative immunology, microbiology and infectious diseases, (2012) (PubMed).

Isling, Aalbaek, Schrøder, Leifsson: "Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology." in: Acta veterinaria Scandinavica, Vol. 52, pp. 48, (2010) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Background: Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway