EIF4E antibody (Eukaryotic Translation Initiation Factor 4E) (AA 170-215)

Details for Product anti-EIF4E Antibody No. ABIN1386772
Antigen
  • 0260/09
  • 0587/11
  • 0589/11
  • 0919/12
  • 1004/13
  • 2
  • 7238
  • CBP
  • CG4035
  • CT13384
  • CT39424
  • CT39426
  • D-eIF4E
  • Dmel\\CG4035
  • Eif4E
  • Eif4e
  • IF4E
  • dEif4e
  • deIF-4E
  • deIF4E
  • eIF 4E
  • eIF-4E1
  • eIF-4E2
  • eIF-4EII
  • eIF-4e
  • eIF4E
  • eIF4E-1
  • eIF4E-1/2
  • eIF4E-2
  • eIF4E1
  • eIF4EI
  • eIF4e
  • eif-4E
  • eif-4e
  • eif4e
  • l(3)07238
  • l(3)67Af
  • l(3)S025007
  • l(3)S026009
  • l(3)S058711
  • l(3)S091912
  • eIF-4E
  • cbp
  • eif4f
  • eif4e1
  • eif4el1
  • AUTS19
  • EIF4E1
  • EIF4EL1
  • EIF4F
  • EG668879
  • Eif4e-ps
  • If4e
  • EIF-4E
  • eif4e-1
  • zgc:86680
  • Eukaryotic initiation factor 4E
  • eucaryotic initiation factor 6
  • eukaryotic translation initiation factor 4E
  • eukaryotic translation initiation factor 4E family member 1B S homeolog
  • eukaryotic translation initiation factor 4E L homeolog
  • eukaryotic translation initiation factor 4ea
  • translation initiation factor eIF4E
  • eIF-4E
  • eif6
  • eif4e
  • eif4e1b.S
  • EIF4E
  • Eif4e
  • eif4e.L
  • eif4ea
  • LOC100533326
Epitope
AA 170-215
80
15
14
13
11
10
8
6
5
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
276
130
128
9
9
8
5
5
5
4
3
3
3
Host
Rabbit
226
52
Clonality
Polyclonal
Conjugate
This EIF4E antibody is un-conjugated
15
11
11
8
8
8
6
6
6
6
6
6
2
2
2
2
2
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
201
124
109
45
42
38
25
18
15
13
13
7
3
1
1
Options
'Independent Validation' Badge
Lot Number 140106
Method validated Immunohistochemistry
Positive Control Human pancreas
Negative Control Human colon
Notes Clear strong signal was detected in positive control sample. Note a minor amount of expected background staining in negative control sample.
Primary Antibody
  • Antigen: Eukaryotic Translation Initiation Factor 4E (EIF4E)
  • Catalog number: ABIN1386772
  • Lot number: 140106
Isotype Control Antibody
  • Antibody: Rabbit IgG isotype control
  • Lot number: C11245
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Lot number: D07640BA
Controls
  • Positive control: Human pancreas tissue stained with antibody
  • Negative control: Human colon tissue stained with antibody
  • Isotype control: Human pancreas tissue stained with isotype control
  • Secondary only control: Human pancreas tissue stained with secondary antibody only
Protocol
  • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with *reaction buffer* x3
  • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iView Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iView DAB/H2O2* applied and incubated for 8 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. *iView Copper* applied and incubated for 4 min
  • 19. Slides rinsed with *reaction buffer*
  • 20. Slides washed in Dawn Detergent/tap water
  • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer (attached).
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
  • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
  • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
Validation Images
Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Figure 1: Human pancreas tissue stained with anti-EIF4E (brown) and counterstained wi...
Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Figure 2: Human colon tissue stained with anti-EIF4E (brown) and counterstained with ...
Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Figure 3: Human pancreas tissue stained with isotype control antibody (brown) and cou...
Immunohistochemistry validation image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Figure 4: Human pancreas tissue stained with secondary antibody only (brown) and coun...
Immunogen KLH conjugated synthetic peptide derived from human eIF4E
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others EIF4E products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Eif4e (EIF4E Antibody Abstract)
Background

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Subcellular location: Cytoplasm

Synonyms: CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Gene ID 1977
UniProt P06730
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-eIF4E Pol...
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 170-215) antibody (ABIN1386772) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
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