Histone H3.3 antibody (pSer31)

Catalog No. ABIN1531501

Product Details anti-Histone H3.3 Antibody

Target: Histone H3.3

Binding Specificity: AA 16-65, pSer31

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Specificity:

Histone H3.3 (Phospho-Ser31) Antibody detects endogenous levels of Histone H3.3 only when phosphorylated at Ser31.

PhosphorylationH:S31 M:S31 R:S31

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31.

Isotype: IgG

Application Details

Application Notes: IF: 1:100~1:500 ELISA: 1:20000

Comment:

Unigene-Number: Hs.180877, Hs.533624, Hs.546259, Hs.699316, Hs.709407 (NCBI Gene Symbol: H3F3B)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Histone H3.3

Target: Histone H3.3

Abstract: Histone H3.3 Products

Background:

Synonyms: CG5825, H3.3Q, H3.A, H3.B, H33, H3F3A, H3F3B, HIS3.3A, HIS3.3B, ORCG8989, HISH3-3Q, Histone H3.3

NCBI Gene Symbol: H3F3B

Molecular Weight: 15 kDa

Gene ID: 3020, 3021

OMIM: 601058

UniProt: P84243