POLR1A antibody (C-Term)

Catalog No. ABIN1536725

Product Details anti-POLR1A Antibody

Target: POLR1A (Polymerase (RNA) I Polypeptide A, 194kDa (POLR1A))

Binding Specificity: AA 1437-1465, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1A antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.

Clone: RB39202

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: POLR1A Antibody (C-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, keep at -20 °C.

Expiry Date: 6 months

Target Details for POLR1A

Target: POLR1A (Polymerase (RNA) I Polypeptide A, 194kDa (POLR1A))

Alternative Name: POLR1A (POLR1A Products)

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).

Molecular Weight: 194811

Gene ID: 25885

NCBI Accession: NP_056240

UniProt: O95602